Bingbing Liu
Zhejiang University,China
Title: A quantitative method for the determination of α-linolenic acid and linoleic acid in chia seed oil by high-performance liquid chromatography
Biography
Biography: Bingbing Liu
Abstract
Alpha-linolenic acid (ALA) and linoleic acid (LA), abundant in chia seed oil, are useful polyunsaturated fatty acids (PUFA) with numerous health benefits. The objective of the present study was to explore the possibilities of the direct analysis of ALA and LA in chia seed oil by reverse-phase HPLC-UV. The results showed that the HPLC-UV method proposed allowed for determining the concentrations of ALA and LA in chia seed oil without applying any isolation or derivatization step. The method was found to be fast (within 18 min), sensitive (LOQ 0.006 mg/ml for ALA and 0.02 mg/ml for LA), and precise (RSD  ≤  2 %). Thus, the proposed experimental designs were shown to offer considerable advantages over traditional derivatization approaches.